BBSRC MRes/PhD Studentship: Regulation of gene expression through coupling of transcription and translation
Doctoral Training Partnership (DTP)
Reference Code: CB073
Name of the supervisors
Dr N Zenkin, Institute for Cell and Molecular Biosciences
Prof K Gerdes, Institute for Cell and Molecular Biosciences
This studentship is sponsored by BBSRC as part of the Doctoral Training Partnership (DTP)
Duration of the award
4 years (MRes Medical and Molecular Biosciences followed by a three year PhD)
Transcription and translation, the two steps of gene expression, in all living organisms are accomplished by highly conserved RNA polymerases and ribosomes. In bacteria and archaea these two processes are coupled and are involved in intricate mutual regulation, which determine the overall rate and quality of gene expression, which, in turn, is important for all cellular processes. Moreover, very recently transcription-translation coupling was proposed to be essential prerequisite of genome stability. However, very little is known about the direct or indirect interactions between the two machineries, and outcomes of these interactions on both, transcription and translation. One of the major reasons for lack of this information was the lack of a suitable in vitro coupled transcription-translation system.
We are the only laboratory in the world that developed a unique experimental system where both steps of gene expression can be assembled from purified components and investigated simultaneously in the test tube. The focus of the project will be to analyze the mutual regulation of the two machineries. We will investigate (i) the contacts between the two machineries, (ii) the effects of coupling on processivity of transcription, transcription pausing and RNA polymerase translocation and (iii) regulation of translation by transcription pausing.
The work involves a uniquely wide range of biochemical, molecular biological and microbiological techniques of investigation of transcription and translation. The laboratory is based in the Centre for Bacterial Cell Biology, which brings together leading scientists in the field of biology of bacterial cell. The Centre is situated in the new building fitted with the state of the art equipment, and provides cutting edge scientific environment.
Value of the Award and Eligibility
Depending on how you meet the BBSRC’s eligibility criteria, you may be entitled to a full or a partial award. A full award covers tuition fees at the UK/EU rate and an annual stipend of £13,590 (2011/12). A partial award covers fees at the UK/EU rate only.
Candidates should have or expect to achieve a Ist class or 2:1 Honours degree in a relevant biological science subject. Previous laboratory experience is essential.
How to Apply
You must complete the University's postgraduate application form. Select "Master of Research/Doctor of Philosophy (Medical Sciences) – Cell and Molecular Biosciences” as the programme of study. Only mandatory fields need to be completed (no personal statement required) but you must attach a copy of your CV and a covering letter, quoting the title of the studentship and reference number CB073.
Closing date for applications
Prompt application is advised as this post is only available until a suitable candidate is appointed
Dr N Zenkin, Nikolay.firstname.lastname@example.org, +44 (0) 191 208 3227